质粒名称: | pLenti X2 Hygro DEST (w17-1) |
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出品公司: | 爱迪基因 |
目录编号: | 17295 |
存储实验室: | Eric Campeau, Paul Kaufman |
载体骨架基本信息 |
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载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体抗性: | 氯霉素和氨苄霉素 |
空载体大小: | 9772 bp |
载体类型: | Lentiviral, RNAi |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | None |
克隆方法: | 限制性内切酶 |
5' 测序引物 | n/a |
文献引用方法 |
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材料和方法部分: | pLenti X2 Hygro DEST (w17-1) was a gift from Vladislav Verkhusha (爱迪基因 plasmid # 17295) |
参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti X2 Hygro DEST (w17-1) 9772 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION 3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Hygro. ACCESSION . VERSION . KEYWORDS pLenti X2 Hygro DEST (w17-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9772) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pLenti X2 Hygro DEST (w17-1)| FEATURES Location/Qualifiers source 1..9772 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 4183..4771 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4236..4256) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4654..4665) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 4827..5326 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(4862..4885) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 5259..5278 /label=mPGK-F /note="Mouse PGK promoter, forward primer" protein_bind 6787..6911 /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(6952..7257) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(7054..7073) /label=ccdB-fwd /note="ccdB gene, forward primer" CDS complement(7599..8258) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 8173..8192 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(8312..8342) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind complement(8367..8491) /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" LTR 8566..8746 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 8818..8939 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8855..8874) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8909..8928 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8958..9093 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9020..9039 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9125..9144) /label=T7 /note="T7 promoter, forward primer" promoter complement(9126..9144) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9154..9171) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9154..9170) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9163..9185) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9315..9770 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9402..9421) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9612..9633 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttccagtgtg 4141 gtggaattct gcagatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac 4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 4561 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 4741 agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc cggactagtg 4801 atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 4861 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 4921 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 4981 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 5041 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 5101 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 5161 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 5221 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 5281 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc 5341 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc 5401 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc 5461 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa 5521 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac 5581 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg 5641 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg 5701 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa 5761 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg 5821 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat 5881 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc 5941 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag 6001 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg 6061 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg 6121 ccgcggctcc ggggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt 6181 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc 6241 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga 6301 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc 6361 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca 6421 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt 6481 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt 6541 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt 6601 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc 6661 cgtcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 6721 ctttttaaaa gaaaaggggg gactggaagg gctagactcg agcggccgcc actgtgctgg 6781 atatcaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 6841 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 6901 cagtcactat ggtcgacctg cagactggct gtgtataacg gagcctgaca tttatattcc 6961 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac 7021 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct 7081 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg 7141 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac 7201 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc 7261 accagtccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag 7321 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct 7381 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata 7441 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct ttttgacata 7501 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca 7561 tactgttatc tggcttttag taagccggat ccagatcttt acgccccgcc ctgccactca 7621 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca 7681 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 7741 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 7801 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 7861 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 7921 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 7981 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg 8041 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 8101 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 8161 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 8221 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 8281 aatctcgccg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta 8341 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt 8401 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 8461 tttctcgttc agcttttttg tacaaacttg ttgatatctg cagaattcca ccacactgga 8521 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta 8581 gaccagatct gagcctggga gctctctggc tgcctaggga acccactgct taagcctcaa 8641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 8701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 8761 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 8821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 8881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 8941 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 9001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 9061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag acgtacccaa 9121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 9181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 9241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 9301 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 9361 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 9421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 9481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 9541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 9601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 9661 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 9721 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cc //